An assertion framework benefits software testing by providing a structured approach to define and verify expected outcomes during the testing process. It allows testers to create assertions that compare actual results against predefined conditions, making it easier to identify discrepancies and bugs. This framework enhances the testing process by promoting clarity, consistency, and reusability of test cases. It can also facilitate automated testing, improving overall efficiency and coverage. Ultimately, assertion frameworks contribute to more reliable and maintainable software by ensuring that quality standards are consistently met.