The ancient civilization that worshipped Osiris as the god of the afterlife was Ancient Egypt. Revered as a deity of resurrection and eternal life, Osiris symbolized regeneration, often associated with the cycles of nature and the annual flooding of the Nile. His myth involved resurrection after being murdered by his brother Seth, emphasizing themes of death, rebirth, and the hope for an afterlife. The beliefs surrounding Osiris influenced Egyptian burial practices and the quest for immortality.